Dihybrid Cross Calculator

Calculate offspring ratios for two-trait crosses using the 9:3:3:1 ratio. Find probabilities of specific genotypes and phenotypes using the product rule and independent assortment.

🧬 Genetics📐 P(AaBb) = P(Aa) × P(Bb)🧬 Biology
Probability of first trait dominant phenotype
Probability of second trait dominant phenotype
Please enter valid values.

Formula & Reference

VariableSymbolFormulaUnits
Dihybrid Cross Calculator—P(AaBb) = P(Aa) × P(Bb)probability

Step-by-Step Examples

Example 1
AaBb x AaBb

Standard dihybrid cross. p1=0.75 (Aa x Aa gives 3/4 dominant), p2=0.75.

  • P(both dominant) = 0.75 x 0.75 = 0.5625 = 9/16
  • P(dom A, rec b) = 0.75 x 0.25 = 0.1875 = 3/16
  • P(rec a, dom B) = 0.25 x 0.75 = 0.1875 = 3/16
  • P(both recessive) = 0.25 x 0.25 = 0.0625 = 1/16
✓ Classic 9:3:3:1 ratio (9/16 : 3/16 : 3/16 : 1/16)
Example 2
AaBB x AaBb

P(dominant A from AaxAa) = 3/4. P(dominant B from BBxBb) = 1.

  • P(A_B_) = 0.75 x 1.00 = 0.75
  • P(A_bb) = 0.75 x 0 = 0
  • P(aaB_) = 0.25 x 1.00 = 0.25
  • P(aabb) = 0
✓ 3:0:1:0 ratio (only B dominant offspring)
Example 3
AABb x aabb

p1 = P(dominant from AA x aa) = 1.0 (all Aa). p2 = P(dominant from Bb x bb) = 0.5.

  • P(A_B_) = 1.0 x 0.5 = 0.5
  • P(A_bb) = 1.0 x 0.5 = 0.5
  • P(aaB_) = 0
  • P(aabb) = 0
✓ 1:1:0:0 ratio

Real-World Applications

Common Mistakes to Avoid

⚠️
Multiply independent probabilities

Product rule: P(A and B) = P(A) x P(B) only if A and B are INDEPENDENT (genes on different chromosomes).

⚠️
Check for linkage first

If genes are linked (same chromosome), probabilities do not multiply independently. Recombination frequency needed.

⚠️
9:3:3:1 is for Aa x Aa for BOTH traits

The classic ratio assumes both parents are diheterozygous (AaBb x AaBb) and genes assort independently.

Frequently Asked Questions

What is independent assortment? ▾
Mendel second law: alleles at one locus assort independently of alleles at another locus (when genes are on different chromosomes). Gives 9:3:3:1 ratio.
What is the product rule in genetics? ▾
P(A and B) = P(A) x P(B) for independent events. Use to find probability of specific offspring genotype from two independent loci.
What is the sum rule? ▾
P(A or B) = P(A) + P(B) for mutually exclusive events. Use when multiple genotypes show same phenotype.
How does linkage change the ratio? ▾
Linked genes produce offspring in parental and recombinant classes. Recombinant frequency (map distance) predicts deviation from 9:3:3:1.
What is a testcross? ▾
Cross unknown genotype with homozygous recessive (aabb). Offspring phenotype ratios directly reveal gamete frequencies of unknown parent.

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